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CELL FREE SYSTEMS > General Cell Free Translation  
 

> Reticulocyte Lysate and Wheat Germ Extracts

 
  > tRNA Crosslinking  
 

> Immunoprecipitation of translation products

 
  > Translation in RRL  
 

> History and evolution of cell-free protein synthesizing systems

 
 

> Import Into Mitochondria

 
 

> Wheat Germ Cell-Free Translation

> Protocol for wheat germ cell-free translation

   

> Wheat Germ Extract Buffers and reagents

   

> Sample WG Protocol

   

> Wheat Germ Extract Recipes

   

> Troubleshooting Wheat Germ Translation Reactions

    > Preparation of a wheat germ extract
  > E coli Translation  

 

WHEAT GERM CELL-FREE TRANSLATION

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4.1 Introduction

As with rabbit reticulocyte lysates (RRL), wheat germ (WG) extracts can be used to program the synthesis of a protein encoded in purified mRNA either native or cell-free transcribed. Read the general introduction to translation for more details.