DWA LAB
Home
Lab Manual
biological reagents
Cell Free Systems
DNA
In Vivo Systems
misc methods
overexpression
recipes
RECIPES > DNA

> CB 10X T4 DNA Ligase (+ATP)

> Plasmid Preps

> TE (Tris-EDTA Buffer)

> Buffering Phenol

> Enzyme Buffers

> DNA gel loading buffer (5 X)

> (TBE) Tris-Borate

> TBE DNA gel running buffer (Tris borate EDTA) 10 X

> Tris-Acetate (TAE)

> Transcription Recipes

 

ENZYME BUFFERS

printer Get a printable version of this protocol (requires Adobe Acrobat

Klenow Fragment: Storage buffer
100mM KH2PO4 (pH 7.0) 556uL of 0.9M
5mM DTT 25uL of 1M
50% glycerol 2.5mL

add some d H2O, check pH, to 5 mL with n H2O

10 X Mung Bean nuclease
0.3 M NaOAc pH 5.0
1 M NaCl
20 mM ZnCl2
 
10 X PHOSPHATASE (calf intestinal phosphatase)
0.2 M Tris 8.0
10 mM MgCl2
10 mM ZnCl2

use 0.1 U CIP to 1 to 20 pmoles DNA ends