RECIPES

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> CB 10X T4 DNA Ligase (+ATP) > Plasmid Preps > TE (Tris-EDTA Buffer) > Buffering Phenol > Enzyme Buffers > DNA gel loading buffer (5 X) > (TBE) Tris-Borate > TBE DNA gel running buffer (Tris borate EDTA) 10 X > Tris-Acetate (TAE) > Transcription Recipes

 

TRIS-ACETATE (TAE)

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1X	For 1L: 50X
0.04 M Tris Ac	242g Tris base
0.002M EDTA	57.1mL glacial acetic acid
	37.2g EDTA
	pH 8.5

50X	For 500mL
2M Tris Ac	121gm Tris base
0.1M EDTA	18.6gm EDTA
	28.5mL glacial acetic acid
	comes to pH 8.5