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CELL FREE SYSTEMS > TRANSLATION > TRANSLATION IN RRL

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      > RPFs from Free and Membrane Bound Ribosomes

 

Sample Protocol

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Translation of preprolactin (#39) at 1/10, 1/20, 1/40, 0 volume of mbs.

T2MM
10uL 80uL
CB 20X 0.5 4.0
E Mix 2.0 16.0
RRL 4.2 33.6
tRNA 0.1 0.8
PIN 0.1 0.8
RNasin 0.1 0.8
CK 0.1 0.8
7uL 56uL

Translation:
  T2 MM #39 T1 mbs H2O Total
A (1/10) 14 2 2 2 20 uL
B (1/20) 14 2 1 3 20 uL
C (1/40) 14 2 0.5 3.5 20uL
D (0) 14 2 - 4 20 uL