Place 200ml-2L of superbroth cell culture into a 500ml polypropylene cetrifuge
bottle(s). Spin at 2800 x g (Beckman JA-10 rotor) for 10 min. Remove supernatant.
Resuspend in 50ml-500ml of Buffer A, spin at 2800 x g (Beckman JA-10 rotor) for
10 min. Remove supernatnat. Repeat.
Add 50ml-500ml of lysis buffer, reususpend cells. Leave on ice 30 min.
Spin for 1.5hours 200,000 x g (Beckman Ti 50.2 rotor) (45 000 rpm).